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Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine

Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine

+38 (050) 554-10-10 +38 (063) 554-10-10 +38 (098) 554-10-10

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Історія лабораторії морфології сполучної тканини

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The laboratory was founded in 1926 at the initiative of the institute’s director, Mykhailo Ivanovych Sytenko, to address fundamental issues in the theory and practice of orthopaedics and traumatology. At that time, the department included the histopathological, bacteriological, and clinical diagnostic sectors. It was headed by the distinguished pathologist Konstantin Fyodorovych Yelenyevsky (1926-1943). Under his leadership, scientific work was carried out, aimed at solving key problems not only in orthopaedics and traumatology but also in the morphology of support structures. Yelenyevsky’s fundamental developments are related to the pathology of metabolism, the study of bone tumours, and bone-joint tuberculosis. From 1943 to 1955, the laboratory was led by Dr. Sci. (Biology) Yevhen Fyodorovych Lysytskyi, a recognized expert in embryonic development, as well as in the regeneration and vascularization of bone and cartilage tissue both in normal conditions and under various pathological processes. For a long time, the laboratory was headed by Prof. Dr. Sci. (Medical) Yevhen Yakovych Pankov (1968-1989). He continued the study of osteoreparation and made a significant contribution to the diagnosis of musculoskeletal tumours. For a short period (1974-1977), the laboratory was led by Nina Mykolayivna Sak. Her research focused on the mechanisms of intervertebral disc degeneration. From 1989 to 2017, the laboratory was headed by Dr. Sci. (Biology) Prof. Ninel Vasylivna Diedukh, a leading expert in the field of musculoskeletal system morphology. Among her main scientific achievements are the development of a new direction for determining the mechanisms of bone remodelling disorders in osteoporosis, its pathogenesis, and the development of osteoporotic fractures, experimental-morphological justification of approaches to the prevention and treatment of osteoporosis, research into bone and cartilage regeneration under the influence of exogenous and endogenous factors, and the use of biomaterials for bone defect replacement, including in the case of osteoporosis. From 2017 to 2024, the laboratory was headed by PhD Natalia Oleksandrivna Ashukina, whose scientific activity was focused on the study of bone tissue regeneration after the influence of various factors, as well as after the implantation of new biomaterials. In collaboration with Prof. Z.Z. Zyman (Kharkiv National University named after V.N. Karazin) and Prof. N.V. Diedukh, she conducted a series of studies on the use of new synthesized types of calcium-phosphate ceramics for osteoplasty in rat experiments, identifying promising samples for clinical use. For 10 years, together with Prof. O.K. Popsuishapka, she researched the causes of non-union in comminuted diaphyseal fractures and methods of their treatment, particularly the role of fibrin clots in bone regeneration for such patients and their practical application in surgical activities. Under her leadership, research was conducted in the field of optimizing bone tissue regeneration using growth factors at various stages of regeneration in combination with mesenchymal cells to improve the repair of critical-size bone defects.

Main Research Areas

  1. Studies of structural and ultrastructural characteristics of skeletal and muscular tissues, namely:
    • investigation of reparative osteogenesis under conditions of endocrine imbalance, exposure to harmful exogenous factors, and the use of medicinal products;
    • histological examination of bone tissue regeneration in the context of implantation of new synthetic materials (corundum and bioactive ceramics, metals with various coatings, carbon, etc.) and biological materials (cultivated skeletogenic cells, allo- and autografts);
    • research into the peculiarities of regeneration of traumatised and degeneratively altered intervertebral discs and articular cartilage;
    • investigation of the mechanisms of osteoporosis and osteoarthritis development, and approaches to correcting such disorders;
    • structural features of muscle tissue in degenerative spinal conditions and the state of muscles under impaired bone regeneration.
  2. Pathoanatomical verification of skeletal and muscular system diseases.
  3. Osteoporosis. Study of bone mineral density in the context of orthopaedic pathology.

The Laboratory of Connective Tissue Morphology is equipped with modern microscopy systems by Olympus (Japan) and Carl Zeiss, providing excellent visualisation and analysis of histological materials using light, polarisation, and fluorescence microscopy techniques. The CellScence software (Olympus, Japan) enables morphometric analysis, photography, and digital preservation of the obtained results.

Bone mineral density studies are carried out using the Explorer QDR W (Hologic) bone densitometer.

Publications by Laboratory Staff

Monographs

Radchenko V. Lumbar facet syndrome / V. Radchenko, N. Dedukh, S. Malyshkina // Modern techniques in spine surgery / Ed. A. Bhave. – New Delhi – London – Philadelphia – Panama: Jaypee Brothers Medical Pub, 2015. – P. 222–232.
Povoroznyuk V.V. Experimental Osteoporosis / V.V. Povoroznyuk, N.V. Dedukh, N.V. Hryhoryeva, I.V. Hopkalova. – Kyiv, 2012. – 228 p.
Guide to Histology / Ed. R.K. Danilov. Vol. 1, Vol. 2. – St Petersburg: Special Literature, 2011. – 832 p.
Osteoarthritis: Conservative Therapy / Eds. N.A. Korzh, N.V. Dedukh, I.A. Zupants. – Kharkiv: Zoloti Storinky, 2007. – 424 p.
Osteoporosis: Epidemiology, Clinical Presentation, Diagnostics, Prevention and Treatment / Eds. N.A. Korzh, V.V. Povoroznyuk, N.V. Dedukh, I.A. Zupants. – Kharkiv: Zoloti Storinky, 2002. – 648 p.

Patents

  1. Pat. 98476 Ukraine. IPC A61B 17/56, G09B 23/28. Method for modelling angular epimetaphyseal deformities of long bones in growing animals / Khmyzov S.O., Yershov D.V., Nikolchenko O.A., Danyshchuk Z.M., Rokutov V.S.; applicant and patent holder – SI “Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine”. – No. u201412859; filed 01.12.2014; published 27.04.2015, Bulletin No. 8.
  2. Pat. 70401 UA, IPC G09B 23/28. Method for modelling osteoarticular tuberculosis in animals / Korzh M.O., Diedukh N.V., Holka T.H. – No. u20111317; filed 21.11.2011; published 10.06.2012, Bulletin No. 11.
  3. Pat. 70401 UA, IPC G09B 23/28. Method for modelling disc herniation / Diedukh N.V. – No. u20111317; filed 21.11.2011; published 10.06.2012, Bulletin No. 11.
  4. Pat. 72359 UA, IPC G09B 23/28. Experimental method for modelling intervertebral disc herniation / Korzh M.O., Radchenko V.O., Shymon M.V., Diedukh N.V., Malyshkina S.V., Chepurnyi V.A. – No. u201203063; filed 16.03.2012; published 10.08.2012, Bulletin No. 15.
  5. Pat. 79708 UA, IPC A61B 10/02 (2006.01), G01N 33/483 (2006.01). Method for predicting the course of bone tissue regeneration / Korzh M.O., Radchenko V.O., Diedukh N.V., Malyshkina S.V., Nikolchenko O.A., Pobel Y.A., Kosterin S.B., Chepurnyi V.A. – No. u201213453; filed 26.11.2012; published 25.04.2013, Bulletin No. 8.
  6. Pat. 60579 UA, IPC A61N 5/067, A61K31/196, A61K31/726 (2006.01). Method of comprehensive conservative treatment for patients with osteoarthritis of the lower limb joints / Korzh M.O., Makolynets V.I., Diedukh N.V., Zupanets I.A., Hrashchenkova T.M., Malyshkina S.V.; filed 22.11.2010; published 25.06.2011, Bulletin No. 12.
  7. Pat. 66537 UA, IPC C25D 11/00. Method for initiating a bactericidal titanium oxide coating on the surface of a titanium implant / Korzh M.O., Radchenko V.O., Diedukh N.V., Malyshkina S.V., Tymchenko I.B., Bilous V.A., Leonov S.O., Khoroshykh V.M., Kholomeyev M.H., Nosov H.I. – No. u201207086; filed 06.06.2011; published 10.01.2012, Bulletin No. 1.
  8. Pat. 92613 Ukraine. IPC A61B 17/56 (2006.01). Method for modelling long bone fractures / Romanenko K.K., Ashukina N.O., Horydova L.D., Prozorovskyi D.V.; applicant and patent holder – SI “Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine”. – No. u201402967; filed 24.03.2014; published 26.08.2014, Bulletin No. 16.

Main publications

  1. Povoroznyuk V., Dedukh N., Makogonchuk A. Effect of aging on fracture healing. Gerontologija, Vilnius. – 2014. – Vol. 15, No. 2. – P. 97–102.
  2. Bengus L.M., Dedukh N.V., Shmanko O.P., Chernyshov A.G., Levshin O.A., Fedotova I.F. Morphological aspects of experimental lumbar spinal stenosis modeling. Inter Medical Journal. – 2014. – No. 1. – P. 102–104.
  3. Umarov F., Diedukh N., Malyshkina S., Khodjaev R. Repair of metadiaphyseal fracture of the humerus in treating animals with calcium preparation in combination with vitamin D3. Medical & Health Science Journal. – 2011. – Vol. 8. – P. 73–79.
  4. Zyman Z., Glushko V., Dedukh N., Malyshkina S., Ashukina N. Porous calcium phosphate ceramic granules and their behaviour in differently loaded areas of skeleton. Journal of Materials Science: Materials in Medicine. – 2008. – Vol. 19(5). – P. 2197–2205.
  5. Malyshkina S.V., Dedukh N.V., Levshin A.A., Kosterin S.B. Modeling of intervertebral disc degeneration in an animal experiment (literature review). Orthopaedics, Traumatology and Prosthetics. – 2015. – No. 1 (598). – P. 114–124.
  6. Radchenko V.A., Kosterin S.B., Dedukh N.V., Pobel E.A. Bone densitometry in clinical practice. Orthopaedics, Traumatology and Prosthetics. – 2015. – No. 2 (599). – P. 100–107.
  7. Skidanov A.G., Ashukina N.O., Danyshchuk Z.M., Batura I.O., Radchenko V.O. Structural features of the multifidus muscle in rats after transpedicular fixation of vertebrae under different motor activity conditions. Orthopaedics, Traumatology and Prosthetics. – 2015. – No. 2 (599). – P. 85–91.
  8. Popsuyshapka A.K., Lytvyshko A.V., Hryhoryev V.V., Ashukina N.A. Treatment of bone fragment nonunion after diaphyseal fracture. Orthopaedics, Traumatology and Prosthetics. – 2014. – No. 1. – P. 34–41.
  9. Tikhonenko A.A., Dedukh N.V., Bengus L.M., Khmyzov S.A. Bone regeneration under the local influence of insulin-like growth factor-1 (experimental study). Orthopaedics, Traumatology and Prosthetics. – 2014. – No. 1. – P. 63–71.
  10. Malyshkina S.V., Zhou L., Dedukh N.V. Structural remodeling of bone tissue in conditions of bone cavity filling with synthetic carbon biomaterial. Orthopaedics, Traumatology and Prosthetics. – 2014. – No. 3. – P. 30–37.
  11. Korzh N.A., Malyshkina S.V., Dedukh N.V., Tymchenko I.B. Biomaterials in orthopaedics and traumatology – the role of A.A. Korzh in the development of the issue. In: Legacy. Oleksii Oleksandrovych Korzh: Scientific and Historical Edition; edited by L.D. Horidova. Kharkiv, 2014. – P. 35–49.
  12. Ashukina N.O., Diedukh N.V., Heleta M.M. Features of reparative osteogenesis in diaphyseal defects of rat femoral bones under hypothyroidism modelling. Tavriia Medical and Biological Bulletin. – 2013. – Vol. 16, No. 1, Part 1 (61). – P. 9–12.
  13. Danyshchuk Z.N., Skidanov I.A., Batura I.A. Morphology of paravertebral muscles in patients with degenerative diseases of the lumbar spine. Tavriia Medical and Biological Bulletin. – 2013. – Vol. 16, No. 1, Part 2. – P. 37–40.
  14. Dedukh N.V., Pobel E.A. Bone tissue in normal conditions and in osteoporosis: calcium and vitamin D preparations (literature review). Orthopaedics, Traumatology and Prosthetics. – 2013. – No. 3. – P. 88–93.