Scientific fields:

• Development of new and introduction of known experimental animal models (rats, rabbits) in order to study in vivo the effects of drugs and physiotherapeutic agents, unhealthy exo- and endogenous factors on the musculoskeletal system under various pathologies
• Experimental testing in vivo on animals (rats, rabbits) to carry out biological evaluation of medical devices (various implant and graft) and to study the course of reparative processes
• Experimental in vitro studies of the effects of various drugs and physiotherapy factors on cultured cells (fibroblasts, chondroblasts, osteoblasts, bone marrow stromal cells); testing in vitro of new implant materials for its cytotoxicity and biocompatibility

Scientific achievements:

About 50 animal models have been developed to study the pathogenesis of various musculoskeletal diseases and the course of reparative processes, including models of osteoarthrosis, osteoporosis, osteochondrosis, scoliosis, intervertebral disc disorders, damage to vertebrae and long bones, etc.

The department performs in vivo and in vitro bioassays of new medical materials and designs, carries out morphometric measurements (somatometry, organometry, osteometry) to quantify the structural changes that occur in the animal body and organs after modeling various pathologies and effect of the studied factors (Certificates of conformity of the measurement system to the requirements of DSTU ISO 10012:2005 «Measurement management system. Requirements for measurement processes and measuring equipment», No. 01-0019/2019 and No. 01-0020/2019 from 2019.02.08).

Experimental biological clinic is equipped with the operating and X-ray rooms. There are 3 veterinarians in the department staff; they provide a skilled level of the experimental studies. The experimental process is organized in accordance with the requirements of the European convention for the protection of vertebrate animals used for experimental and other scientific purposes (Strasbourg, 1986), «Good Laboratory Practice» (SТ-G МHU 42-6.0: 2008), national standard DSTU ISO 10993 «Biological evaluation of medical devices». Certified equipment and compliance with international standards ISO 13485-2005, ISO 9001-2009 allow the department to work in the fundamental and clinical directions with bone allogenic implants and stem cell cultures (according to CMU Resolution No. 695 from 2000.04.24).

The department scientific and practical activities are associated with all departments of the Sytenko Institute as well as with other domestic and foreign institutes, universities and clinics (including researches supported by STCU grants – projects # 3174, # 4231).

The department published 6 monographs, 25 patents for invention, 165 articles, 2 methodical recommendations, 15 innovations during the past 20 years.

Priority publications of the Department (2020–2025)

Articles

1. Taran A. V., Vorontsov P. M., Makarov V. B., Nikol’chenko O. A., Gnidenko Yu. P., Romaniuk S. P., Tymoshenko O. I., Misiruk I. O. (2025). Functional ZrTaN coatings for acetabular component of hip joint endoprosthesis. Problems of Atomic Science and Technology, 1 (155), 113–116. https://doi.org/10.46813/2025-155-113
2. Taran A. V., Garkusha I. E., Nowakowska-Langier K., Tolmachova G. N., Vorontsov P. M., Nikol’chenko O. A., Gnidenko Yu. P., Romaniuk S. P., Tymoshenko O. I., & Misiruk I. O. (2024). Microstructure, chemical composition, and nanomechanical properties of ARC-PVD ZrTa/ZrTaO2 bilayer coatings. Problems of Atomic Science and Technology, 4 (152), 132–135. https://doi.org/10.46813/2024-152-132
3. Pavlichenko R. O., Klosovskij A. V., Kulik Yu. S., Misiruk I. O., Taran A. V., Vorontsov P. M., Gnidenko Yu. P., & Nesterenko Ye. O. (2024). Portable ozone generating device for treatment of infected wounds. Problems of Atomic Science and Technology, 6 (154), 96–99. https://doi.org/10.46813/2024-154-096
4. Makarov V.B., Dedukh N.V., & Nikolchenko O.A. (2023). Features of bone remodeling around surface-modified titanium and tantalum implants. Wiadomości Lekarskie Medical Advances, 76 (8), 1790–1796. https://doi.org/10.36740/WLek202308113
5. Bondarenko S., Filipenko V., Ashukina N., Maltseva V., Ivanov G., Lazarenko Iu., Sereda D., & Schwarzkopf R. (2023). Comparative study in vivo of the osseointegration of 3D-printed and plasma-coated titanium implants. World Journal of Orthopedics, 14 (9), 682–689. 10.5312/wjo.v14.i9.682
6. Ashukina N., Maltseva V., Vorontsov P., Danyshchuk Z., Nikolchenko O., & Korzh M. (2022). Histological evaluation of the incorporation and remodeling of structural allografts in critical size metaphyseal femur defects in rats of different ages. Romanian Journal of Morphology and Embryology, 63 (2), 349–356. https://doi.org/10.47162/RJME.63.2.06
7. Rogulska O.Y., Trufanova N.A., Petrenko Y.A., Repin N.V., Grischuk V.P., Ashukina N.O., Bondarenko S.Y., Ivanov G.V., Podorozhko E.A., Lozinsky V.I., & Petrenko A.Y. (2022). Generation of bone grafts using cryopreserved mesenchymal stromal cells and macroporous collagen‐nanohydroxyapatite cryogels. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 110 (2), 489–499. https://doi.org/10.1002/jbm.b.34927
8. Radchenko V., Ashukina N., Maltseva V., Skidanov M., Nikolchenko O., Danyshchuk Z., & Skidanov A. (2022). Models of paraspinal muscle degeneration in rats: high-fat diet and prolonged compression. Wiadomości Lekarskie Medical Advances, 75 (9, pt. 2), 2280–2285. 10.36740/WLek202209218
9. Vyrva O.Ye., Holovina Ya.O., Malyk R.V., Danishchuk Z.M., Ashukina N.O., & Vorontsov P.M. (2021). Surgical treatment of bone tumors using segmental bone allografts. Zaporozhye Medical Journal, 23 (1), 159–164. https://doi.org/10.14739/2310-1210.2021.1.224965
10. Taran V.S., Garkusha I.E., Vorontsov P.M., Boldyriev I.N., Gnidenko Yu.P., Klosovskij A.V., Taran A.V., & Lozina A.S. (2021). Development of multi-purpose portable air ozonator based on surface DBD. Problems of Atomic Science and Technology. Series: Plasma Physics (27), 131 (1), 114–116. https://doi.org/10.46813/2021-131-114
11. Lozina A.S., Garkusha I.E., Shypilov S.A., Vorontsov P.M., Bolduriev I.N., Gnidenko Y.P., Taran A.V., & Chechelnitckij O.G. (2021). Multifunctional DBD-Based Ozone Sterilizer with Ultrasonic Cavitation for Low-Temperature Treatment of Medical Tools. Plasma Medicine, 11 (2), 55–61. https://doi.org/10.1615/PlasmaMed.2021039629
12. Bondarenko S., Filipenko V., Karpinsky M., Karpinska O., Ivanov G., Maltseva V., Badnaoui A.A., & Schwarzkopf R. (2020). Osseointegration of porous titanium and tantalum implants in ovariectomized rabbits: a biomechanical study. World Journal of Orthopedics, 12 (4), 214–222. 10.5312/wjo.v12.i4.214
13. Taran A., Lozina A., Garkusha I., Taran V., Krasnyj V., Gnidenko Y., Vorontsov P., Starikov V., & Mamalis A. (2021). Evaluation of active power in dielectric barrier discharge with pulse power supply. Journal of Instrumentation, 16 (12). https://doi.org/10.1088/1748-0221/16/12/T12019
14. Bondarenko S., Ashukina N., Maltseva V., Ivanov G., Badnaoui A.A., & Schwarzkopf R. (2020). Evaluation of the bone morphology around four types of porous metal implants placed in distal femur of ovariectomized rats. Journal of Orthopaedic Surgery and Research, 15 (296), 7. 10.1186/s13018-020-01822-3
15. Shymon V., Ashukina N., Maltseva V., Alfeldiy S., Shymon M., Savvova O., & Nikolchenko O. (2020). Bone repair after the glass-ceramics implantation into the rats’ femur defect. Georgian Medical News, (3), 105–111.
16. Kaminska M., Dihtiar V., Dedukh N., & Nikolchenko O. (2020). Reactive-adjustable restructuring of sternum in rats after modeling of mechanical loading in the biomechanical system “sternum-ribs-spine”. Georgian Medical News, (5), 140–147.
17. Taran V.S., Garkusha I.E., Taran A.V., Muratov R.M., Vorontsov P.M., Gnidenko Yu.P., Herasimov H.M., Starikov V.V., Baturin A.A., & Romaniuk S.P. (2020). Functional protective ZrN coatings on implants for trauma surgery. Problems of Atomic Science and Technology, 6 (130), 115–118. https://doi.org/10.46813/2020-130-115

Abstracts of reports

Makarov V., Dedukh N., Nikolchenko О. Bone Remodeling Around Titanium Implants With Through Pores And Implants With Rough Surfaces. 26th EFORT Annual Congress. Lyon, 11–13 June 2025. Аbstract #714

Makarov V., Dedukh N., Nikolchenko O. Revision hip arthroplasty with 3D-printed custom porous titanium cup: from histological and morphometric study to clinical outcome. 15th Congress of the European Hip Society. Bern, 12-13 October 2023. Р. 180. Poster EHS23-2188.

Makarov V., Dedukh N., Nikolchenko О. Polylactide Implants With An Emphasis On The Bone. 20th EFORT Annual Congress. Lisbon, 05–07 June 2019. Аbstract #3430

1. Makarov V., Dedukh N., Nikolchenko O. Osteointegration of the Implants with Diamond-like Carbon Coatings. 1st Virtual EFORT Congress. 28–30 October 2020. Аbstract #1652

Korzh M., Garkusha I., Vorontsov P., Nikolchenko O., Ashukina N., Babych O., Taran А., Starikov V., Gnidenko Ju., Misiruk I. Biocompatibility of the surgery polypropylene mesh implants with zircon coatings: experimental study. Annual Meeting of the German Society for Biomaterials. Stuttgart, 7–8 October 2021. P. 97. URL: https://www.dgbm-kongress.de/fileadmin/congress/media/dgbm2021/ondemand/DGBM_2021_Abstractband.pdf

Korzh M., Vorontsov P., Ashukina N., Nikolchenko O., Maltseva V., Danyshchuk Z., Velyaminova V., Samoilova K. Bone allograft in critical size defect rebuilding with an emphasis on the age: an experimental histological study. Annual Meeting of the German Society for Biomaterials. Stuttgart, 7–8 October 2021. P. 98. URL: https://www.dgbm-kongress.de/fileadmin/congress/media/dgbm2021/ondemand/DGBM_2021_Abstractband.pdf

2. Korzh M., Makarov V., Dedukh N., Bondarenko S., Nikolchenko O. Bone remodeling around titanium 3D-implants with through and rough surfaces in experiment. Annual Meeting of the German Society for Biomaterials. Stuttgart, 7–8 October 2021. P. 99. URL: https://www.dgbm-kongress.de/fileadmin/congress/media/dgbm2021/ondemand/DGBM_2021_Abstractband.pdf
3. Radchenko V., Ashukina N., Maltseva V., Skidanov M., Nikolchenko O., Danyshchuk Z., Skidanov A. (2022). Two experimental rat models for studying degenerative changes in the lumbar paravertebral muscles. Aging Clinical and Experimental Research, (34, Suppl. 1), S. 312 (Р631). DOI: 10.1007/s40520-022-02147-3 (Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases. Berlin, 24–26 March 2022)